Alternatively, a short powerful throw may reduce aggregation of cells. Shear-sensitive cultures may need a larger throw to provide good mixing while minimising the chance of damaging the cells. How good is dissolved oxygen transfer?Īn orbital shaker mechanism provides the best mixing and aeration for both microbial and cell cultures. Liquid collection, wipeable surfaces and antimicrobial coating all have a part to play.ħ. What can I do about spills and the potential for contamination?Ī way to prevent spillages from damaging critical components and keeping contamination ricks to a minimum is key for any incubator shaker. A stacking system for plates can greatly increase the capacity of each deck of an incubator shaker, allowing several thousand individual experiments to be conducted simultaneously.Ħ. What do I need for rapid screening applications?Īny type of culture in standard 96-well microwell plates may need a combination of high speed and very short throw (3 mm) to ensue good mixing. Stackable units increase capacity for a given footprint.ĥ. A small incubator shaker may be located under a laboratory bench to save work space. Take this into account when working out where the incubator shaker can be located in your laboratory. How much room do I have available and where?Īll incubator shakers need a clear area around them for good gas and heat exchange. Keep in mind that a lower filling volume in a shake flask (10-15 % rather than 20-25 %) will greatly increase the surface area to volume ratio for improved gas transfer.Ĥ. The filling volume of individual vessels will largely determine the overall height and weight of the load. High, heavy loads will lead to reduced shaker speeds and must be distributed evenly over the tray to avoid uneven loads that may cause vibrations or limit the performance of the shaker. What are the height and weight of my proposed load? Universal sticky mats allows for direct contact or sticking a suitable holder in place.ģ. This basic information is essential for ordering a suitable tray. What type, quantity and size of culture vessels do I need? Not all options will be needed in all cases. The optimised growth of different types of organism depend on the capabilities provided by the incubator shaker. What will be cultured in the incubator shaker? Points to consider when buying an incubator shaker:ġ.
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